希伯来大学案例：3D水凝胶中的单细胞封装与培养
简介
此研究由以色列希伯来大学的Ram实验完成。
他们基于微流体、分子学、细胞生物学和计算生物学等科学技术，从单细胞水平去研究RNA-seq 和 ChIP-seq。微流体驱动方面，他们信赖法国Fluigent，采购了十几台Flow EZ压力泵和流量计。
相关文献：
[1]. Li, H. et al. Reference component analysis of single-cell transcriptomes elucidates cellular heterogeneity in human colorectal tumors. Nat. Genet. 49, 708–718 (2017).
[2]. Dalerba, P. et al. Single-cell dissection of transcriptional heterogeneity in human colon tumors. Nat. Biotechnol. 29, 1120–1127 (2011).
[3]. Kim, K. T. et al. Single-cell mRNA sequencing identifies subclonal heterogeneity in anti-cancer drug responses of lung adenocarcinoma cells. Genome Biol. 16, 1–15 (2015).
[4]. Tirosh, I. et al. Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq. Science (80-. ). 352, 189–196 (2016).
[5]. Bavli, D. et al. CloneSeq: A highly sensitive analysis platform for the characterization of 3D-cultured single-cell-derived clones. Dev. Cell (2021).
相关实验室网站：
https://ramoren3.wixsite.com/oren-ram-lab
https://www.bio.huji.ac.il/en/departments_biological_chemistry_en
010-58237080
